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Download Animal Cell Technology: Basic & Applied Aspects: Proceedings by Hiroshi Nakato, Susumu Izumi, Scott B. Selleck (auth.), Y. PDF

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By Hiroshi Nakato, Susumu Izumi, Scott B. Selleck (auth.), Y. Kitagawa, T. Matsuda, S. Iijima (eds.)

Animal mobilephone expertise is a turning out to be self-discipline of mobile biology which goals not just to appreciate buildings, capabilities and behaviors of differentiated animal cells but in addition to envision their skills for use for commercial and scientific reasons. The objective of animal phone know-how contains accomplishments of clonal enlargement of differentiated cells with priceless skill, optimization in their tradition stipulations, modulation in their skill for construction of medically and pharmaceutically very important proteins, and the appliance of animal cells to gene remedy and synthetic organs. This quantity supplies the readers an entire evaluate of the current state-of-the-art in Japan. The complaints might be important for phone biologists, biochemists, molecular biologists, immunologists, biochemical engineers and different disciplines regarding animal telephone tradition, operating both in educational environments or in industries of biotechnology and pharmacy.

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Read or Download Animal Cell Technology: Basic & Applied Aspects: Proceedings of the Tenth Annual Meeting of the Japanese Association for Animal Cell Technology, Nagoya, Japan, November 5–8, 1997 Volume 10 PDF

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Extra info for Animal Cell Technology: Basic & Applied Aspects: Proceedings of the Tenth Annual Meeting of the Japanese Association for Animal Cell Technology, Nagoya, Japan, November 5–8, 1997 Volume 10

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To test whether ST3Gal IV or ST3Gal III is involved in the biosynthesis of the oligosaccharides, we compared the levels of the antigens on transfected Namalwa KJM-1 cells by fluorescence activated cell sorter (FACS) using monoclonal antibodies (mAbs), CSLEX-1 and KM93. The levels of the antigens were considerably increased by expression of ST3Gal IV, but not by that of ST3Gal III. 2. 1. 3. Expression Levels of ST3Gal III and ST3Gal IV mRNAs in Various Cells Using competitive reverse transcriptase-polymerase chain reaction (RT-PCR) analyses, we examined the expression levels of ST3Gal IV and ST3Gal III mRNAs in a variety of cells including polymorphonuclear cells (PMN), monocytes, their immortalized cell lines (HL-60, U-937, and THP-1), and colon carcinoma cell lines (Colo205, SW1116, and LSI80), which were known to express the antigens.

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