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Extra resources for (NAS Colloquium) Genetic Engineering of Viruses and Viral Vectors

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As compared with the maximum cell viability (100%), reductions in alamarBlue dye conversion produced by the virus-induced cell lysis were generated as a function of the multiplicity of infection. All the human glioma cell lines tested were sensitive to the cytotoxic effects of the engineered HSV. 5 altered viruses required >1000-fold more virus for cell killing of human astrocytes obtained from normal human cerebral cortical tissue. A series of in vivo experiments were performed to establish the effects of these genetically engineered viruses.

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