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By Pawlicki, Todd
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Use analgesics and anesthetics for potentially painful procedures. Perform surgeries and procedures aseptically. Perform only a single major surgery on any one animal whenever possible. Provide appropriate postsurgical care. S. Congress directed the National Institutes of Health (NIH) to develop a plan for implementing the three Rs. The NIH responded by producing a document entitled “Plan for the Use of Animals in Research,” which provides an overview of biomedical models for research, including animals, and charges investigators with the responsibility for selecting the optimum approach to their research.
Intraperitoneal and intravenous injections are the most common routes for injecting substances in rodents, and they are discussed in detail in the following sections. 4 Transportation In most instances, animals will need to be moved or relocated to a facility that houses imaging equipment. Transport, even from the simplest move of animals from one room to another, can alter basic physiological functions, such as elevated heart rate and blood pressure. Consideration of potential stress induced by transport between facilities, between rooms within a facility, and between holding areas and imaging areas should be given.